Journal: Journal of Cell Science

Article Title: Reduced vacuolar ATPase protects mice from Friend virus infection – an unintended but instructive effect in Hif-2a fl mice

doi: 10.1242/jcs.261893

Figure Lengend Snippet: Mice with a floxed exon 2 of Hif-2a are resistant to FV infection. (A,B) Spleen weight (A) and viral load (B) of different mouse strains were analyzed 7 days after FV infection. Mice designated as Hif-2a fl in B and C are littermate Hif-2a fl × LysM +/+ mice. For reasons of clarity the designation has been shortened. ‘ Hif-2a fl total’ represents the combined results of all different Hif-2a fl mouse strains. (C) The number of erythroblasts (Ter119 + ), macrophages (F4/80 + ) and B cells (CD19 + ) was determined by flow cytometry. Results are mean±s.e.m. The numbers in the graphs indicate the number of animals tested. * P <0.05; ns, not statistically significant (compared to naïve mice; one-way ANOVA and Tukey's multiple comparison test).

Article Snippet: Briefly, the bone marrow was flushed from the bone cavity with a 23 G needle and syringe (BD Biosciences; Heidelberg, Germany) containing macrophage medium [minimal essential medium, MEM, plus 100 U/ml penicillin/streptomycin, 5 mM L-glutamine, 1% (v/v) sodium pyruvate (all from Invitrogen, Waltham, MA, USA), 1 mM HEPES (Sigma-Aldrich, Munich, Germany) and 0.6 mM tissue culture grade β-mercaptoethanol (Merck KGaA, Darmstadt, Germany) and 10% (v/v) fetal calf serum (FCS; Biochrom GmbH, Berlin, Germany)].

Techniques: Infection, Flow Cytometry, Comparison